| Product Name | VE(Vitamin E) ELISA Kit | Species | Universal |
|---|---|---|---|
| Uniprot ID | N/A | Sensitivity | 0.88 pg/mL |
| Standard | 100pg/mL | Detection Range | 1.56-100pg/mL |
| Sample type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids, Sample volume: 50μL | ||
| Reaction time | 1.5H | Research Area | Metabolic pathway; Signal Transduction; Cancer, Nutrition metabolism;Hormone metabolism; Signal transduction;Metabolic pathway; Enzyme & Kinase; Signal transduction;Metabolic pathway; Nutrition metabolism;Hormone metabolism;Cardiovascular?Signaling transd |
| Test Principle | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VE. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to VE. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VE in the samples is then determined by comparing the OD of the samples to the standard curve. |
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