| Product Name |
VB6(Vitamin B6) ELISA Kit |
Species |
Universal |
| Uniprot ID |
N/A |
Sensitivity |
1.42 ng/mL |
| Standard |
300 ng/mL |
Detection Range |
4.69-300 ng/mL |
| Sample type |
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids, Sample volume: 50μL |
| Reaction time |
1.5H |
Research Area |
Nutrition metabolism;Hormone metabolism; |
| Test Principle |
| This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VB6. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to VB6. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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