| Product Name | Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit | Species | Rat |
|---|---|---|---|
| Uniprot ID | P07632 | Sensitivity | 0.1 ng/mL |
| Standard | 10ng/mL | Detection Range | 0.16-10ng/mL |
| Sample type | Serum, Plasma, Tissue homogenate and Other biological samples?Sample Volume=100μL | ||
| Reaction time | 1.5H | Research Area | Human gene deletion; |
| Test Principle | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat SOD1. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Rat SOD1. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SOD1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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