| Product Name |
Mouse GRO Alpha/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit |
Species |
Mouse |
| Uniprot ID |
P12850 |
Sensitivity |
9.38 pg/mL |
| Standard |
1000pg/mL |
Detection Range |
15.63-1000pg/mL |
| Sample type |
Serum, Plasma, Tissue homogenate and Other biological samples?Sample Volume=100μL |
| Reaction time |
3.5H |
Research Area |
Signal transduction;Cardiovascular biology;Reproductive science;Neuro science; |
| Test Principle |
| This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GRO?/CXCL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GRO?/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GRO?/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GRO?/CXCL1. You can calculate the concentration of Mouse GRO?/CXCL1 in the samples by comparing the OD of the samples to the standard curve. |
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