| Product Name |
Human Beta-EP(Beta-Endorphin) ELISA Kit |
Species |
Human |
| Uniprot ID |
P01189(237~267aa) |
Sensitivity |
9.38 pg/mL |
| Standard |
1000pg/mL |
Detection Range |
15.63-1000pg/mL |
| Sample type |
Serum, Plasma, Tissue homogenate and Other biological samples?Sample Volume=100μL |
| Reaction time |
1.5H |
Research Area |
Metabolic pathway; |
| Test Principle |
| This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human ?-EP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Human ?-EP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ?-EP in the samples is then determined by comparing the OD of the samples to the standard curve. |
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