| Product Name | 3-NT(3-Nitrotyrosine) ELISA Kit | Species | Universal |
|---|---|---|---|
| Uniprot ID | N/A | Sensitivity | 1.35 ng/mL |
| Standard | 300 ng/mL | Detection Range | 4.69-300 ng/mL |
| Sample type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other biological fluids, Sample volume: 50μL | ||
| Reaction time | 1.5H | Research Area | Metabolic pathway;Infection immunity;Rheumatology; |
| Test Principle | This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 3-NT. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 3-NT. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve. |
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